The mechanisms of carbon catabolite repression in bacteria. Barba C., Mart M., Roddick-Lanzilotta A., Manich A., Carilla J., Parra J.L., Coderch L. Effect of wool keratin proteins and peptides on hair water sorption kinetics. As the medium have both glucose and lactose as carbon sources, E. coli shall utilize glucose first through carbon catabolite repression mechanism until it is exhausted followed by utilization of lactose as the carbon source [26]. This can be done in the same way as a figure or table by referring to the video or animation content and noting in the body text where it should be placed. Langbein L., Yoshida H., Praetzel-Wunder S., Parry D.A., Schweizer J. Web references As a minimum, the full URL should be given and the date when the reference was last accessed. The graphical abstract should summarize the contents of the article in a concise, pictorial form designed to capture the attention of a wide readership. Diameter of the hairs before and after treatment with K31 was measured by inverted microscope (Nikon Eclipse TS 100). These will be used instead of standard icons and will personalize the link to your video data. M9NG medium [15] was used for expression of keratin K31. Chemical treatment of hair, such as bleaching, causes damage by penetrating deep within the hair fiber and cleaving keratin K31, which results in decreased tensile strength. As these lack complete sequence identity with human hair keratin, are likely to be less efficient than the human hair keratin. Do not include footnotes in the Reference list. We are always looking for ways to improve customer experience on Elsevier.com. This combination of letters and format will enable Elsevier's typesetters to recognise the relevant texts as accession numbers and add the required link to GenBank's sequences.Example: GenBank accession nos. Note: since video and animation cannot be embedded in the print version of the journal, please provide text for both the electronic and the print version for the portions of the article that refer to this content. There are no abstract, keywords or section headings. To facilitate reproducibility and data reuse, this journal also encourages you to share your software, code, models, algorithms, protocols, methods and other useful materials related to the project. When coding terminology is used, we recommend to avoid offensive or exclusionary terms such as "master", "slave", "blacklist" and "whitelist". Hair, being one of the most important components of the beauty care processes, attracts the use of a variety of hair treating cosmetics. If an author has moved since the work described in the article was done, or was visiting at the time, a 'Present address' (or 'Permanent address') may be indicated as a footnote to that author's name. Abstract A concise and factual abstract is required (maximum length 250 words). Keep the layout of the text as simple as possible. The ePub format uses eBook readers, which have several "ease of reading" features The neutralizing agent was then washed out using a commercial shampoo [25]. Elsevier collaborates with a number of repositories to link articles on ScienceDirect with relevant repositories, giving readers access to underlying data that gives them a better understanding of the research described. An error in a letter or number can result in a dead link. Data references should include the following elements: author name(s), dataset title, data repository, version (where available), year, and global persistent identifier. We advise to seek gender neutrality by using plural nouns ("clinicians, patients/clients") as default/wherever possible to avoid using "he, she," or "he/she." The eluted fractions containing the desired protein, as shown by SDS-PAGE analysis, were pooled. Electronic artwork General points Make sure you use uniform lettering and sizing of your original artwork. For comparison, unbleached hairs were used as a control. Do not include references to personal communications, unpublished data or manuscripts either 'in preparation' or 'submitted for publication'. Preferred file types: TIFF, EPS, PDF or MS Office files. The [dataset] identifier will not appear in your published article. Most hair cosmetic products and hair treatments initially affect the cuticle, changing the appearance, softness and even texture of hair [10]. When copying references, please be careful as they may already contain errors. Bhm G., Muhr R., Jaenicke R. Quantitative analysis of protein far UV circular dichroism spectra by neural networks. If no funding has been provided for the research, it is recommended to include the following sentence: Units Follow internationally accepted rules and conventions: use the international system of units (SI). View full aims & scope. Please supply 'stills' with your files: you can choose any frame from the video or animation or make a separate image. Keratin from chicken feather or other animal sources extracted using chemical methods, either in the intact form or its hydrolyzed product have been used in the commercial cosmetic products [1]. Number them consecutively throughout the article. Conflict of Interest All authors must disclose any financial and personal relationships with other people or organizations that could inappropriately influence (bias) their work. Subdivision - numbered sections Divide your article into clearly defined and numbered sections. For more information, please review the Elsevier Policy on the Use of Images or Personal Information of Patients or other Individuals. Both the naturally damaged hair and those partially damaged by bleach treatment showed gain in their diameter after keratin treatment. Contributors Each author is required to declare their individual contribution to the article: all authors must have materially participated in the research and/or article preparation, so roles for all authors should be described. Authors should fully disclose all posting in registries of results of the same or closely related work. The manuscript should be in line with the Recommendations for the Conduct, Reporting, Editing and Publication of Scholarly Work in Medical Journals and aim for the inclusion of representative human populations (sex, age and ethnicity) as per those recommendations. The datasets will be listed and directly accessible to readers next to your published article online. These findings show the potential of K31 in the development of hair care and styling products. The molecular weight of these keratins range from 46 to 55kDa. However, do use bold face, italics, subscripts, superscripts etc. Superscript Arabic numerals are used for such footnotes. 1). Keywords Immediately after the abstract, provide a maximum of 6 keywords, using British spelling and avoiding general and plural terms and multiple concepts (avoid, for example, 'and', 'of'). Protein disulphide isomerase-mediated grafting of cysteine-containing peptides onto over-bleached hair. MALDI-TOF mass spectroscopy showed the characteristics expected for human K31 keratin. Concise and informative. Reference style References should be numbered consecutively (with parentheses) as they appear in the text. The mechanical strength of the bleached hair increased by almost 2 fold after a single treatment of K31. In particular, do not use the word processor's options to justify text or to hyphenate words. The CD was calibrated with 0.05M Tris-Cl (pH 7.5) before loading the sample. DNA encoding Keratin K31 (Gene ID: 3881) was synthesized in pEX-K cloning vector (Eurofins Genomics, Wolverhampton, UK) along with NdeI and EcoRI restriction sites at the 5 and 3 end, respectively. already built in. Supply captions separately, not attached to the figure. Please note that your manuscript will be independently reviewed by the new journal. Tensile strength of the naturally damaged and the bleach-treated hair showed marked improvement after keratin treatment. Referees It is required that Authors provide a list of 4 or 5 potential reviewers (e-mail and phone number) who are knowledgeable in the subject matter, have no conflict of interest, and are likely to agree to review the manuscript. This work was carried out in the laboratories of School of Biological Sciences, University of the Punjab, Pakistan. SDS PAGE analysis of proteins in E. coli cells expressing keratin K31. Authors who have video or animation files that they wish to submit with their article are strongly encouraged to include links to these within the body of the article. Research data refers to the results of observations or experimentation that validate research findings. More information on types of peer review. Biotechnol Rep (Amst). However, the intact protein in its native state can be more effective in forming a cohesive film by filling the gaps produced in the damaged hair [24]. Graphical abstracts should be submitted as a separate file in the online submission system. BE675048 ), and a T-cell lymphoma (GenBank accession no. Application of K31 to the chemically damaged hairs resulted in increase of mechanical strength more than twice to 26N. It appears that keratin K31, the major protein lost in the damaged hair [11], gets integrated in to the damaged hair filling the gaps and thus enhancing their elasticity and mechanical strength. It takes a lot of washing to get out. T7 promoter based pET-22b (+) was used as the expression vector (Novagen, Madison, USA). Coldspring Harbour. It makes your hair strands stiff and sticky. The Tryptic digest thus obtained was analyzed by MALDI-TOF mass spectrometry. Examples of potential conflicts of interest include employment, consultancies, stock ownership, honoraria, paid expert testimony, patent applications/registrations, and grants or other funding. The CONSORT checklist and template flow diagram are available online. If you have any problems submitting your paper through this system, please contact the Editorial Office on: e-mail: jgar@elsevier.com; tel: +44 (0)1865 843270; fax: +44 (0)1865 843992. Types of paper The following types of manuscripts are routinely accepted (please note that word count is from abstract to references but excluding references): Original Articles: The form of these articles is discussed fully below; an abstract is required. molecular epidemiology of resistant organisms; drug-resistant viral emergence in treated and untreated patients; microbiological and clinical studies stewardship; antimicrobial resistance testing detection and diagnostic; viral resistant variants and impact on public health; PK/PD parameters and prediction of efficacy; surveillance studies of resistant pathogens affecting humans isolated from different hosts and environments. Yamaguchi H., Miyazaki M. Refolding techniques for recovering biologically active recombinant proteins from inclusion bodies. the display of certain parts of an article in other eReaders. Each spectrum obtained was the average of two consecutive scans with a 1nm bandwidth. Open access Please visit our Open Access page for more information. The keratins of the human beard hair medulla: the riddle in the middle. Present the authors' affiliation addresses (where the actual work was done) below the names. (b) Mass spectroscopic analysis of the tryptic digest of keratin K31. keratin K31-K40. Please complete and upload this form (pdf version or word version) to declare funding, conflict of interest and to indicate whether ethical approval was sought. 1. The alkali treated hair sample, however, when washed with plain water retained its curly form. When preparing tables, if you are using a table grid, use only one grid for each individual table and not a grid for each row. The protein, however, was expressed as insoluble inclusion bodies. Please submit your material together with the article and supply a concise, descriptive caption for each supplementary file. See also the section on illustrations.To avoid unnecessary errors you are strongly advised to use the spellchecker.The title page should include: the title, the name(s) and affiliation(s) of the author(s), an address for correspondence, and telephone/fax numbers for editorial queries. In the case of addition or removal of authors, this includes confirmation from the author being added or removed.Only in exceptional circumstances will the Editor consider the addition, deletion or rearrangement of authors after the manuscript has been accepted. Essential title page information Title.

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